Optimization of Agrobacterium tumefaciens-mediated Transformation in Oxalis corniculata (L.)

Background: Optimization of co-cultivation parameters during Agrobacterium-mediated transformation to Oxalis corniculata evaluated were bacterial density, infection period, acetosyringone (AS) concentration and temperature. Five fold efficiency is achieved in transient gus gene expression after optimizing various parameters. The optimized conditions Agrobacterium tumefaciens growth phase A600nm 0.17, period 30 min, addition medium (400 μM) cocultivation temperature 22°C. Methods: (L.) used for transformation. Bacterial culture was added 50 ml liquid YEP with kanamycin rifampicin grown until reaching the (A600nm). density ranged from 0.56,0.80 1.34 OD present study. made solid MS consisted BAP 2 mg L-1 NAA 0.5 concentrations AS at 0, 50, 100, 200, 400,600 800 μM. Histochemical analysis carried out. Result: Higher resulted more efficiency, but also higher necrosis explants. Dilution suspension reduced explants 64% when process out μM). Our studies proved that among conditions, critical mediated